期刊
TALANTA
卷 214, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.120842
关键词
Aggregation-induced emission; Nano-sensors; Hypochlorite; Living cells and zebrafish
资金
- National Natural Science Foundation of China [81972394, 31800833]
- Science and Technology Planning Projects of Guangdong Province [2016A020215175]
- Natural Science Foundation of Guangdong Province [2016A030313583]
- Medical Scientifc Research Foundation of Guangdong Province [A2016555]
- Outstanding Youths Development Scheme of Nanfang Hospital, Southern Medical University [2015J005]
- Science and Technology Planning Project of Guangzhou [201704020070]
Hypochlorite (ClO-) could be used as a diagnostic marker for inflammation and related diseases. Although there have been many reports on probes for ClO- imaging, there was still a lack of specificity and anti-interference ability. Herein, carbazole (NEC) and tetraphenylethylene (TPE) equipped with thiobarbituric acid (TBA), NEC-TBA and TPE-TBA, were synthesized and used as a fluorescence biosensor for monitoring ClO- with aggregation-induced emission (AIE) effect. we identified that TPE-TBA, with formed nanoparticles in the mean grain size at 76 nm (5 mu M), was a superior probe to target ClO- over other analytes with fluorescence turn off strategy. Subsequently, to explore the bioimaging application, TPE-TBA was able to sense exogenous ClO- in living HeLa cells through fluorescence imaging. In zebrafish model, TPE-TBA effectively captured exogenous ClO- in the entire organization of zebrafish. Overall, these AIE-based probes merit further development as organism targeting ClO- sensors.
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