期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 237, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.118381
关键词
Fluorescent probe; Carbon dots; RNA detection; Nucleolus imaging
类别
资金
- National Natural Science Foundation of China [21974125, 21708035]
- Foundation for University Key Teacher by Henan Province [2017GGJS007]
- Key Scientific Research Project in Universities of Henan Province [19A150048]
- China Postdoctoral Science Foundation [2017M620302, 2018T110736]
RNA as a carrier of genetic information plays a critical role in various physiological processes. RNA-rich nucleolus is usually employed as an important biomarker for many malignant diseases. Herein, RNA-responsive fluorescent carbon dots (CDs) were synthesized by a simple microwave method. Due to the presence of cationic benzothiazolium groups in the CDs, a turn-on fluorescence signal was achieved between CDs and RNA. The CDs exhibit excellent RNA selectivity and a good linear relationship with a detection limit of 0.62 mu g/mL. The small particle size, polarity sensitivity and RNA response behavior of CDs realized fast and wash-free nucleolus imaging effectively. Overall, these CDs provide a powerful potential tool for monitoring cell nucleus activity and elucidating RNA dynamics. (C) 2020 Elsevier B.V. All rights reserved.
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