期刊
SMALL
卷 16, 期 32, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202002801
关键词
anodic aluminum oxide; core-shell SERS nanotags; point of care testing; vertical flow assays
类别
资金
- National Key Research and Development Program of China [2018YFF0215200]
- Fundamental Research Project of Shenzhen Science & Technology Innovation Committee [JCYJ20180306174552939]
There is a great demand for the development of detection assays for inflammation infection diagnosis with high throughput and ultrasensitivity. Herein, a vertical flow assay system with functionalized nanoporous anodic aluminum oxide (AAO) as sensing membrane, and encoded core-shell surface enhanced Raman scattering (SERS) nanotags as labels for multiple inflammatory biomarkers detection is presented. A 2 x 2 test array on the porous AAO is developed and modified with multiple capture antibodies to capture inflammatory biomarkers from samples. Due to the high surface area to volume ratio of the AAO membrane, and its influence on plasmonic coupling, the electromagnetic field of the encoded core-shell SERS nanotags is enhanced. Detection limits of 53.4, 4.72, 48.3, and 7.53 fg mL(-1)are realized for C reactive protein, interleukin-6, serum amyloid A, and procalcitonin, respectively, with a linear dynamic range spanning at least five orders of magnitude. In addition, the proposed method also shows acceptable accuracy and repeatability for the detection of clinical samples. Therefore, this approach is expected to be a powerful point of care testing tool for disease diagnosis in facility limited areas.
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