期刊
SEPARATION AND PURIFICATION TECHNOLOGY
卷 255, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.seppur.2020.117538
关键词
Cyanobacteria; Anabaena cylindrica; C-phycocyanin; Chlorophylls; Sequential extraction; Purification; Aqueous biphasic systems; Stability
资金
- Portuguese Foundation for Science and Technology (FCT)/MCTES [UIDB/50011/2020, UIDP/50011/2020, UIDP/50017/2020 + UIDB/50017/2020]
- I&D SusPhotoSolutions [CENTRO-01-0145-FEDER-000005]
- FCT [SFRH/BD/122220/2016, SFRH/BD/123850/2016]
- national funds (OE), through FCT I.P.
- [PTDC/BTA-BTA/30914/2017]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/123850/2016, PTDC/BTA-BTA/30914/2017] Funding Source: FCT
This study presents a sequential downstream process for extracting C-phycocyanin and chlorophyll from Anabaena cylindrica. By optimizing operational conditions, high pigment recovery rates were achieved. Additionally, purification of C-phycocyanin using polymer-based ABS led to a significant increase in purity without compromising photostability.
Cyanobacteria are attracting the attention worldwide as a reliable and sustainable feedstock for the production of biofuels, food colorants and biochemically active compounds. Anabaena cylindrica (A. cylindrica) is a cyanobacterium with a significant widespread occurrence in Portuguese freshwaters, with C-phycocyanin and chlorophylls, compounds with a high market value, being abundantly produced by the species. This work presents the development of a sequential downstream process to obtain C-phycocyanin and chlorophyll from A. cylindrica. The C-phycocyanin extraction is carried using Na-phosphate (20 mM, pH 7) buffer, while aqueous solutions of surface-active compounds (250 mM), as well as ethanol, were screened for the extraction of the chlorophylls. After selecting the best solvents to recover both pigments from A. cylindrica , the operational conditions (solid-liquid ratio, time of extraction and temperature) were optimized, allowing the recovery of around 90% and 55% of the total content of C-phycocyanin and chlorophylls, respectively. Finally, dextran/ copolymer-based ABS were applied to promote the purification of C-phycocyanin from the residual chlorophylls and other contaminant proteins, leading to an increase in the C-phycocyanin purity of 4-fold, without compromising its photostability.
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