期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 316, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128131
关键词
Au@CDs; Enhanced ECL; Circulating tumor cells; Separation-free; Label-free
资金
- National Natural Science Foundation of China [21707053]
- Advanced Talents Science Foundation of Jiangsu University [10JDG052, 10JDG038]
In this work, new gold@carbon dots nanoalloys (Au@CDs) were synthesized by directly heating carbon dots (CDs) and HAuCl4 aqueous solution to boiling. The as-synthesized Au@CDs exhibited 12-fold enhancement in the ECL emission compared to the single CDs. The high luminous efficiency Au@CDs was further used to fabricate an ECL cytosensor for the direct detection of circulating tumor cells (CTCs) in serum by ligation of a cell-specific aptamer. Briefly, human mucin1 protein (MUC1) aptamer was immobilized onto the surface of Au@CDs on the electrode for capturing MUC1-positive MCF-7 cells (a type of CTCs). On the basis of the increased steric hindrance, the captured MCF-7 cells can be quantitatively detected. Our strategy was free from complicated separation and labeling treatment, and realized the detection of MCF-7 cells (a type of CTCs) from 100 to 10,000 cells/mL with a detection limit of 34 cells/mL, offering promising applications in ultrasensitive routine clinical analysis and point-of-care testing.
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