期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 317, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128213
关键词
Fluorescent probe; Sulfur-substituted coumarin; Hypochlorite; Desulfurization; Fluorescence imaging
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A3A2048814, 2019R1A6A1A11044070]
- National Research Foundation of Korea [2019R1A6A1A11044070] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A thiocoumarin-based turn-on fluorescence probe for the detection of hypochlorite anion (ClO-) was prepared and characterized. Coumarin dye (CZCN-O) exhibited intense fluorescence with good quantum yields (Phi(PL) = 0.98) in acetonitrile. Contrastingly, the fluorescence of thiocoumarin dye (CZCN-S) was almost completely quenched (Phi(PL)< 0.01) because of the efficient intersystem crossing from the emissive singlet excited state to the non-emissive triplet state. The addition of ClO- to the PBS buffer (pH = 7.4, containing 50% ACN) of thiocoumarin resulted in a remarkable fluorescence enhancement with an emission maximum at 486 nm, indicating the sensitive OFF-ON fluorescence sensing of ClO-. The calculated detection limit of ClO- in 50% aqueous ACN was 0.17 mu M. An investigation of the sensing behavior to various analytes revealed that thiocoumarin dye had an excellent selectivity for ClO-. The recognition mechanism was attributed to the ClO--induced conversion of thiocarbonyl coumarin into its oxo analogue through oxidative desulfurization. In particular, cell studies demonstrated that thiocoumarin dye could be used as an effective fluorescent probe for the detection of ClO- in living cells.
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