期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 323, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128673
关键词
Two-photon fluorescence; Biothiols and H2S; Turn-on Probe; Bioimaging; ROS
资金
- National Natural Science Foundation of China [81672508, 61601218]
- Jiangsu Provincial Foundation for Distinguished Young Scholars [BK20170041]
- Department of Science and Technology of Shaanxi Province [2020GXLH-Z-008, 2020GXLH-Z-023]
- Northwestern Polytechnical University [2020GXLH-Z-008, 2020GXLH-Z-023]
- Natural Science Basic Research Program of Shaanxi [2019JM-016]
- Key Research and Development Program of Shaanxi [2020ZDLGY13-04]
- Open Research Fund of Anhui Key Laboratory of Tobacco Chemistry [20181140]
- China-Sweden Joint Mobility Project [51811530018]
- Fundamental Research Funds for the Central Universities
Biothiols and sulfides, especially glutathione (GSH), cysteine (Cys), homocysteine (Hcy) and hydrogen sulfide (H2S) play important roles in an extensive range of biological processes and functions. Due to their reductivity, they are deemed as second messengers of oxidative stress, and the imbalance of these biothiols are closely associated to a variety of diseases. Herein, we report a two-photon (TP) turn-on probe DT-01 that can detect biothiols and sulfides both in vitro and in vivo. The probe was synthesized by nitrobenzoxadiazole (NBD) derivative (NBD-Cl) and pyrimidine-based TP fluorophore (FL-01), the fluorescence of DT-01 is quenched due to the photoinduced charge transfer (PET) from FL-01 moiety to NBD moiety. Upon substitution reaction by nucleophilic biothiols, TP fluorophore released and exhibited turn-on fluorescence at 550 nm. We further demonstrated that DT-01 can detect biothiols and H2S in living cells, mice brain slices and zebrafish. More importantly, by real-time detecting the total content of biothiols and H2S, we have successively measured the changes of oxidative stress levels in HeLa cells.
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