Distinct mechanisms underlying the assembly of microeukaryotic generalists and specialists in an anthropogenically impacted river

Microeukaryotic communities are sensitive to environmental changes; and are considered essential formicrobial foodwebs and biogeochemical cycles. Therefore, understanding the community responses of microeukaryotes to environmental changes is of great ecological significance. Very little is known about the assembly mechanisms underlying the microeukaryotic communities, especially for the key ecological groups (e.g., habitat generalists/specialists) in the riverine ecosystems. Here, we employed 18S rDNA amplicon sequencing to study the assembly processes governing the microeukaryotic communities and their habitat generalists and specialists across three hydrological seasons in a subtropical river in China. The results showed that deterministic and stochastic processes jointly shaped the microeukaryotic communities, where the relative importance of stochastic processes decreased in the following order: wet > normal > dry seasons. However, deterministic processes played more important role in shaping the microeukaryotic communities than those of prokaryotes. Meanwhile, stochastic and deterministic processeswere responsible for structuring themicroeukaryotic habitat generalists and specialists, respectively. Generally, the pure effects of physicochemical factors on themicroeukaryotic communities and their key ecological groups were similar to 1.7 folds than those of the micropollutants. However, several micropollutants (e.g., acetaminophen, benzophenone-3, bisphenol A, sulfadiazine, triclocarban and triclosan) were found to have a strong effect on the habitat specialists. Altogether, we suggested that the ecological responses of the riverine microeukaryotes to micropollutants may vary among species due to the intrinsic differences in their environmental plasticity. (c) 2020 Elsevier B.V. All rights reserved.