期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 750, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.141694
关键词
Antibiotic resistance genes; Dairy farm; Soil resistome; Soil reservoir; Calf; Antibiotic treatment
资金
- Agricultural Animal Health Program, College of Veterinary Medicine
- Washington State Agricultural Research Center
- National Natural Science Foundation of China [31872597]
- Jiangsu Agricultural Science and Technology Independent Innovation Fund [CX (18)2012-02]
- Peter J. Shields Endowed Chair in Dairy Food Science
Exposure to antibiotic-treated animals had a direct impact on the soil microbiome and resistome, leading to significant increases in antimicrobial resistance genes (ARGs) in the exposed soil. Evaluating on-farm effects to soil after exposure to antibiotic-treated animals can help in designing effective management strategies to mitigate antibiotic resistance in food-animal production.
We determined the immediate impact of exposure to antibiotic-treated animals on housing soil microbiome and resistome. Fecal (n = 36) and soil (n = 108) samples from dairy calves (n = 6) treated with and without florfenicol over 30 days were collected. There were temporary changes in the gut microbiome of antibiotic-treated calves as measured by Shannon diversity (16S rRNA gene sequencing; P = 0.03), but not in the housing soil microbiome (P > 0.05). Droplet-digital PCR demonstrated that floR gene increased by 1-log in soil exposed to treated animals (P < 0.001), but it remained relatively stable in the control soil whereby calves were not treated with antibiotic. Resistome in exposed soil was largely modified (P = 0.004) with the overall prevalence of antimicrobial resistance genes (ARGs) significantly elevated (3.8-fold increase by day 10; P = 0.01). In addition to florfenicol, enriched ARGs collectively conferring resistance to tetracyclines, aminoglycosides, sulfonamides, elfamycins, macrolides-lincosamides-streptrogramin A/B, and beta-lactams. Quantitative PCR validated that ARGs including str and tetG in soil exposed to florfenicol-treated calves had gradually increased fold-change difference relative to the control soil over time. Moreover, a greater diversity of transferrable ARGs was observed in exposed soil and these were associated with a greater diversity of bacterial species. Evaluation of on-farm effects to soil in situ after exposure to antibiotic-treated animals can help design effective managements to mitigate antibiotic resistance in food-animal production. (C) 2020 Elsevier B.V. All rights reserved.
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