期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 725, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.138367
关键词
As(III) exposure; Agricultural soil; Antibiotic resistance; As(III) resistance; Potential pathogen
资金
- National Program for Support of Top-Notch Young Professionals, Guangdong Special Support Program [2017TX04Z351]
- Science and Technology Program of Guangzhou, China [201903010071]
The increased acquisition of antibiotic resistance by pathogens is a global health concern. The environmental selection of antibiotic resistance can be caused by either antibiotic residues or co-selecting agents such as toxic metal(loid)s. This study explored the potential role of As(III) as a co-selecting driver in the spread of antibiotic resistance in paddy soils. By applying high-throughput sequencing, we found that the diversity and composition of soil microbial communities was significantly altered by As(III) exposure, resulting in an increased proportion of potential pathogens (9.9%) compared to the control soil (0.1%). Meanwhile, a total of 46 As(III)-resistant isolates were obtained from As(III)-exposure soil, among which potential pathogens accounted for 54.3%. These As(III)-resistant bacteria showed a high incidence of resistance to sulfanilamide (100%) and streptomycin (88-93%). The association between antibiotic and As(III) resistances was further investigated in a potentially pathogenic isolate by whole-genome sequencing and a transcription assay. The results showed that As(III) and antibiotic resistance genes might co-occur in a mobile genomic island and be co-regulated by As(III), implying that antibiotic resistance could be co-selected by As(III) via co-resistance and co-regulationmechanisms. Overall, these results suggest that As(III) exposure provides a strong selective pressure for the expansion of soil bacterial resistome. (C) 2020 Elsevier B.V. All rights reserved.
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