期刊
REPRODUCTION IN DOMESTIC ANIMALS
卷 55, 期 11, 页码 1526-1534出版社
WILEY
DOI: 10.1111/rda.13802
关键词
apoptosis; embryo development; oocyte maturation; porcine; small ubiquitin-like modifier; UBC9
资金
- National Natural Science Foundation of China [31960659]
- Natural Science Foundation of Jilin Province [20190301037NY]
SUMOylation is a dynamic post-translational modification process. However, the function of small ubiquitin-like modifiers (SUMOs) in the maturation of porcine oocytes and embryo growth is not well known. Therefore, the aim of this study was to investigate the effect of E2 binding enzyme UBC9 on the expression of SUMO-1 protein during the in vitro maturation of porcine oocytes and embryo development after in vitro fertilization. Four groups were used: 0 (Control), 5, 10 and 15 mu g/ml UBC9. Western blotting, flow cytometry and RT-qPCR were used to detect the in vitro maturation of porcine oocytes, SUMO-1 content, viability and the expression of apoptotic genes. Compared to those in the control treatment, the maturation rate (p < .05) and viability (p < .01) of oocytes in the 5 mu g/ml treatment group decreased significantly. SUMO-1 protein markers appeared at 59 and 71 kDa and the content of SUMO-1 protein in the 10 mu g/ml treatment group decreased significantly (p < .05). In the expression of apoptosis-related genes, Bcl-2 gene expression was significantly downregulated in the 10 mu g/ml treatment group (p < .05). However, Bax and Caspase-3 were significantly upregulated in the 5 mu g/ml treatment group (p < .05). During embryonic development, the cleavage rate of oocytes in the 10 mu g/ml treatment group was significantly reduced (p < .05), whereas blastocyst formation rate in the 5 mu g/ml treatment group was significantly reduced. UBC9 regulates SUMO-1 content in mature pig oocytes in vitro, which affects oocyte maturation rate, viability, apoptotic genes expression and embryo development after fertilization.
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