期刊
RENEWABLE ENERGY
卷 153, 期 -, 页码 522-529出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2020.01.151
关键词
Butanol; Hydrogen; Food waste; Fermentation; Amylase; Clostridium
资金
- National Research Foundation, Prime Minister's Office, Singapore under the Competitive Research Program [NRF-CRP5-2009-05]
- Ministry of Education, Singapore under Academic Research Fund [R-302-000-148-112]
This study developed a process using an amylolytic Clostridium sp. strain BOH3 to produce butanol and hydrogen from food waste without enzymatic pretreatment. Strain BOH3, which possesses genes encoding amylases, can produce 14.1 g/L butanol and 16.2 mmol hydrogen from 180 g/L food waste. Protein sequence analysis shows that the amylase in Clostridium sp. strain BOH3 may contain more active sites and possess a higher translation rate than that in Clostridium beijerinckii NCIMB 8052. After calcium was confirmed able to promote the activities of the amylase, the butanol production was improved to 16.6 g/L and the hydrogen production was increased to 18.2 mmol from 180 g/L food waste when external calcium ions were supplemented. Compared to a previous similar study (12.5 g/L), the butanol production is 1.3 - fold of that production. Butanol productivity was also enhanced to 0.17 g/L/h due to the shorter fermentation duration caused by enhanced amylase activities. Thus, Clostridium sp. strain BOH3 is a promising candidate for one-step butanol production using food waste to mitigate environmental sustainability issues. (C) 2020 Elsevier Ltd. All rights reserved.
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