期刊
CLINICAL ORAL INVESTIGATIONS
卷 21, 期 5, 页码 1659-1665出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00784-016-1963-4
关键词
Growth factor; Bone morphogenetic protein-9; Bone formation; Collagen; Osteoinduction
资金
- Japan Society for the Promotion of Science (JSPS) [24792147, 26462972]
- Grants-in-Aid for Scientific Research [24792147, 26462972] Funding Source: KAKEN
It has been reported that bone morphogenetic protein (BMP)-9 has potent osteoinductive properties among the BMP family by adenovirus-transfection experiments. We very recently reported that absorbable collagen sponge (ACS) as a carrier for recombinant human (rh) BMP-9, compared with chitosan sponge, was suitable for inducing bone healing/regeneration by BMP-9 in a rat calvarial defect model. The aim of this study was to evaluate different doses of rhBMP-9/ACS on new bone formation in rat critical size calvarial defects. Bilateral calvarial defects (n = 32) were surgically created in 16 wistar rats and randomly filled with one of the following materials: (1) absorbable collagen sponge (ACS) alone; (2) 1 mu g-rhBMP-9/ACS (L-rhBMP-9/ACS); (3) 5 mu g-rhBMP-9/ACS (H-rhBMP-9/ACS); and (4) blank defects (control). The animals were sacrificed 8 weeks postsurgery for radiographic and histomorphometric analyses. Bone volume and defect closure were statistically higher in the rhBMP-9/ACS-implanted (L-rhBMP-9/ACS and H-rhBMP-9/ACS) groups when compared with ACS-alone group (p < 0.05). Furthermore, defects filled with H-rhBMP-9/ACS showed the highest levels of newly formed bone area (NBA) and NBA/total defect area among all groups. No significant differences in any of the radiographic and histometric parameters could be observed between both concentrations of rhBMP-9. Within the limits of this study, it can be concluded that rhBMP-9/ACS-induced bone formation can be reached with as little as 1 mu g/site in rat critical size calvarial defects. RhBMP-9 could be a potential therapeutic growth factor for future bone regenerative procedures.
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