期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 31, 页码 18849-18857出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2004183117
关键词
autoinhibited calcium ATPase; calcium; flg22; vacuole
资金
- National Science Foundation [MCB1329723, MCB1614965, IOS1557899]
- National Aeronautics and Space Administration [NNX13AM50G, NNX14AT25G, 80NSSC19K0126]
- Japan Science and Technology Agency PRESTO
- Japan Society for the Promotion of Science KAKENHI [17H050077, 18H04775, 18H05491]
- University of Wisconsin-Madison Graduate School
- National Science Foundation
- Grants-in-Aid for Scientific Research [18H04775, 18H05491] Funding Source: KAKEN
One of the major events of early plant immune responses is a rapid influx of Ca2+ into the cytosol following pathogen recognition. Indeed, changes in cytosolic Ca(2+ )are recognized as ubiquitous elements of cellular signaling networks and are thought to encode stimulus-specific information in their duration, amplitude, and frequency. Despite the wealth of observations showing that the bacterial elicitor peptide flg22 triggers Ca2+ transients, there remain limited data defining the molecular identities of Ca2+ transporters involved in shaping the cellular Ca2+ dynamics during the triggering of the defense response network. However, the autoinhibited Ca2+ -ATPase (ACA) pumps that act to expel Ca2+ from the cytosol have been linked to these events, with knockouts in the vacuolar members of this family showing hypersensitive lesion-mimic phenotypes. We have therefore explored how the two tonoplast-localized pumps, ACA4 and ACM11, impact flg22-dependent Ca2+ signaling and related defense responses. The double-knockout aca4/11 exhibited increased basal Ca2+ levels and Ca2+ signals of higher amplitude than wild-type plants. Both the aberrant Ca2+ dynamics and associated defense-related phenotypes could be suppressed by growing the aca4/11 seedlings at elevated temperatures. Relocalization of ACA8 from its normal cellular locale of the plasma membrane to the tonoplast also suppressed the aca4/11 phenotypes but not when a catalytically inactive mutant was used. These observations indicate that regulation of vacuolar Ca2+ sequestration is an integral component of plant immune signaling, but also that the action of tonoplast-localized Ca2+ pumps does not require specific regulatory elements not found in plasma membrane-localized pumps.
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