期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 25, 页码 14150-14157出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2001806117
关键词
xanthophyll; P450; hydroxylation; chirality; stereospecificity
资金
- National Natural Science Foundation of China [31670794, 31530006]
- National Key R&D Program of China [2017YFA0503703]
- Chinese Academy of Sciences [XDB17030100]
- Chang Jiang Scholars Program [Q2017241]
- Anhui Provincial Wanjiang Scholars Program
Two cytochrome P450 enzymes, CYP97A3 and CYP97C1, catalyze hydroxylations of the beta- and epsilon-rings of alpha-carotene to produce lu- tein. Chirality is introduced at the C-3 atom of both rings, and the reactions are both pro-3 R-stereospecific. We determined the crys- tal structures of CYP97A3 in substrate -free and complex forms with a nonnatural substrate and the structure of CYP97C1 in a detergent -bound form. The structures of CYP97A3 in different states show the substrate channel and the structure of CYP97C1 bound with octylthioglucoside confirms the binding site for the carotenoid substrate. Biochemical assays confirm that the ferredoxin- NADP + reductase (FNR) -ferredoxin pair is used as the redox part- ner. Details of the pro -3 R stereospecificity are revealed in the retinal -bound CYP97A3 structure. Further analysis indicates that the CYP97B clan bears similarity to the beta-ring -specific CYP97A clan. Overall, our research describes the molecular basis for the last steps of lutein biosynthesis.
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