A novel plant protein-disulfide isomerase participates in resistance response against the TYLCV in tomato

Main conclusion Overexpression or silencing of theSlPDIcould increase plants resistance or sensitivity to TYLCV through enhancing or reducing the plant's antioxidant capacity. Tomato yellow leaf curl virus(TYLCV), a plant virus that could infect a variety of crops, is particularly destructive to tomato growth. Protein disulfide isomerase (PDI) is a member of the thioredoxin (Trx) superfamily, is capable of catalyzing the formation and heterogeneity of protein disulfide bonds and inhibiting the aggregation of misfolded proteins. Studies have shown that PDI plays important roles in plant response to abiotic stress, there is no research report on the function of PDI in response to biotic stress, especially TYLCV infection. Here, we identified a tomato PDI gene,SlPDI, was involved in regulating tomato plants resistance to TYLCV. Subcellular localization results showed that SlPDI was located at the endoplasmic reticulum (ER), and its location remained unchanged after infection with TYLCV virus. Overexpression or silencing ofSlPDIcould increase plants resistance or sensitivity to TYLCV. Transgenic plants that overexpressingSlPDIexhibit enhanced antioxidant activity evidenced by lower hydrogen peroxide (H2O2) level and higher activity of superoxide dismutase (SOD) and peroxidase (POD) in comparison with WT plants, after infected by TYLCV. Moreover, theSlPDI-silencing plants showed opposite results. The promoter analyzes result showed thatSlPDIwas involved in response to salicylic acid (SA), and our experimental results also showed that the expression level ofSlPDIwas induced by SA. Taken together, our results indicated that SlPDI could regulate plant resistance to TYLCV through enhancing the protein folding function of ER and promoting the synthesis and conformation of antioxidant-related proteins.