期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 151, 期 -, 页码 650-658出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2020.03.039
关键词
Wheat bZIP TFs; ABA biosynthesis; TaCDPK9-1; TabZIP8, 9, 13; TaNCED1, 2
资金
- National Natural Science Foundation of China [31660392]
- Northwest Normal University [2019GG-3, NWNU-LKQN-14-11]
In plants, basic leucine zipper (bZIP) transcription factors (TFs) participate in various biological processes such as development and stress responses. But the molecular mechanism of wheat bZIP TFs modulating abscisic acid (ABA) biosynthesis is unknown. In this study, we demonstrated the expressions of three bZIP TF genes TabZIP8, 9, 13, were regulated by Triticum aestivum calcium (Ca2+-dependent protein kinase 9-1 (TaCDPK9-1) and they took part in ABA biosynthesis in wheat roots under salt stress. We first isolated TabZIP8, 9, 13 and TaCDPK9-1 from wheat. TabZIP8, 9, 13 genes transcripts were strongly induced by salt stress, but salt-induced TabZIP8, 9, 13 transcriptions were drastically impaired by Ca2+ channel blocker LaCl3. TaCDPK9-1 kinase could interact with TabZIP8, 9, 13 TFs through yeast two-hybrid assay. Next, the expression levels of salt-induced wheat 9-cis-epoxycarotenoid dioxygenasel, 2 (TaNCED1,2) encoding a key enzyme controlling ABA production and salt-induced ABA content were found to be decreased under LaCl3 treatment, and yeast one-hybrid experiment revealed TabZIP8, 9, 13 could bind to the ABA-responsive elements (ABREs) and the promoter sequence of TaNCED2 gene. Together, our results suggest that salt stress-induced ABA accumulation is mediated by TabZIP8, 9, 13, which are adjusted by TaCDPK9-1 in wheat roots.
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