期刊
PLANT CELL AND ENVIRONMENT
卷 43, 期 9, 页码 2254-2271出版社
WILEY
DOI: 10.1111/pce.13813
关键词
drought; leaf growth; maize; photosynthesis; proteomics; sh2mutant; starch
资金
- Interuniversity Attraction Poles Program (Belgian Network MARS: Growth and Development of Higher Plants) [IUAP VII/29]
- Science Policy Office of the Belgian State
- University of Antwerp
- Research Foundation - Flanders (FWO) fellowship [12U8918N]
To understand the growth response to drought, we performed a proteomics study in the leaf growth zone of maize (Zea maysL.) seedlings and functionally characterized the role of starch biosynthesis in the regulation of growth, photosynthesis and antioxidant capacity, using theshrunken-2mutant (sh2), defective in ADP-glucose pyrophosphorylase. Drought altered the abundance of 284 proteins overrepresented for photosynthesis, amino acid, sugar and starch metabolism, and redox-regulation. Changes in protein levels correlated with enzyme activities (increased ATP synthase, cysteine synthase, starch synthase, RuBisCo, peroxiredoxin, glutaredoxin, thioredoxin and decreased triosephosphate isomerase, ferredoxin, cellulose synthase activities, respectively) and metabolite concentrations (increased ATP, cysteine, glycine, serine, starch, proline and decreased cellulose levels). Thesh2mutant showed a reduced increase of starch levels under drought conditions, leading to soluble sugar starvation at the end of the night and correlating with an inhibition of leaf growth rates. Increased RuBisCo activity and pigment concentrations observed in WT, in response to drought, were lacking in the mutant, which suffered more oxidative damage and recovered more slowly after re-watering. These results demonstrate that starch biosynthesis contributes to maintaining leaf growth under drought stress and facilitates enhanced carbon acquisition upon recovery.
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