期刊
PHYTOCHEMISTRY
卷 174, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2020.112349
关键词
Lotus japonicus; Leguminosae; Biosynthesis; Strigolactone; 5-Deoxystrigol; 18-Hydroxylcarlactonoic acid; Lotuslactone; Cytochrome P450 (CYP); Lotus japonicus MORE AXILLARY GROWTH 1 (MAX1)
资金
- Program for Promotion of Basic and Applied Research for Innovations in BioOriented Industry
- Japan Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry
- JSPS [17J05519]
- Grants-in-Aid for Scientific Research [17J05519] Funding Source: KAKEN
Strigolactones (SLs) are a group of plant apocarotenoids that act as rhizosphere signaling molecules for both arbuscular mycorrhizal fungi and root parasitic plants. They also regulate plant architecture as phytohormones. The model legume Lotus japonicas (synonym of Lotus corniculatus) produces canonical 5-deoxystrigol (5DS) and non-canonical lotuslactone (LL). The biosynthesis pathways of the two SLs remain elusive. In this study, we characterized the L. japonicas MAX1 homolog, LjMAX1, found in the Lotus japonicas genome assembly build 2.5. The L. japonicas maxi LORE1 insertion mutant was deficient in 5DS and LL production. A recombinant LjMAX1 protein expressed in yeast microsomes converted carlactone (CL) to 18-hydroxycarlactonoic acid (18-OH-CLA) via carlactonoic acid (CLA). Identity of 18-OH-CLA was confirmed by comparison of the methyl ester derivative of the MAX1 product with chemically synthesized methyl 18-hydroycarlactonoate (18-OH-MeCLA) using LCMS/MS. (11R)-CL was detected as an endogenous compound in the root of L. japonicas. C-13-labeled CL, CLA, and 18-OH-MeCLA were converted to [C-13]-5DS and LL in plant feeding experiments using L. japonicas WT. These results showed that LjMAX1 is the crucial enzyme in the biosynthesis of Lotus SLs and that 18-hydroxylated carlactonoates are possible precursors for SL biosynthesis in L. japonicas.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据