Description of an atypicalToxoplasma gondiiisolate from a case of congenital toxoplasmosis in northeastern Brazil

We report a case of severe congenital toxoplasmosis that involved an atypicalT. gondiigenotype in a newborn baby from Alagoas state in Northeastern Brazil. A pregnant woman presented IgM and IgG anti-T. gondiiantibodies, as detected by the chemiluminescence immunoassay on the second trimester of pregnancy. A mouse bioassay was performed using umbilical cord blood and one isolate was obtained. The isolate was designated TgCTBrAL1 and genetic characterization revealed genotype ToxoDB #162. Genotype results of the rhoptry genes, ROP5 and ROP18, could predict the high virulence of the isolate in mice, which was confirmed by an in vivo virulence assay. This is the first report of generating aT. gondiiisolate from a newborn baby with congenital toxoplasmosis in Northeastern Brazil.