期刊
PARASITOLOGY
卷 147, 期 11, 页码 1171-1183出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S003118202000092X
关键词
mRNA decay; Pumilio; translation; Trypanosoma brucei
类别
资金
- Deutsches Akademisches Austauschdienst (DAAD)
- Deutsche Forschungsgemeinshaft [CL112/24]
Trypanosomes strongly rely on post-transcriptional mechanisms to control gene expression. Several Opisthokont Pumilio domain proteins are known to suppress expression when bound to mRNAs. TheTrypanosoma bruceiPumilio domain protein PUF3 is a cytosolic mRNA-binding protein that suppresses expression when tethered to a reporter mRNA. RNA-binding studies showed that PUF3 preferentially binds to mRNAs with a classical Pumilio-domain recognition motif, UGUA[U/C]AUU. RNA-interference-mediated reduction of PUF3 in bloodstream forms caused a minor growth defect, but the transcriptome was not affected. Depletion ofPUF3also slightly delayed differentiation to the procyclic form. However, bothPUF3genes could be deleted in cultured bloodstream- and procyclic-form trypanosomes. Procyclic forms without PUF3 also grew somewhat slower than wild-type, but ectopic expression of C-terminally tagged PUF3 impaired their viability. PUF3 was not required for RBP10-induced differentiation of procyclic forms to bloodstream forms. Mass spectrometry revealed no PUF3 binding partners that might explain its suppressive activity. We conclude that PUF3 may have a role in fine-tuning gene expression. Since PUF3 is conserved in all Kinetoplastids, including those that do not infect vertebrates, we suggest that it might confer advantages within the invertebrate host.
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