期刊
ORAL DISEASES
卷 27, 期 3, 页码 439-447出版社
WILEY
DOI: 10.1111/odi.13565
关键词
Alix; exosome; oral squamous cell carcinoma; tumor marker
资金
- Japan Society for the Promotion of Science [26221304, JP17H04065, JP18K17025]
- Ministry of Education, Culture, Sports, Science and Technology: Nanotechnology Platform
This study found that exosomal Alix levels in serum/saliva were significantly higher in patients with OSCC compared to healthy controls, indicating potential diagnostic biomarkers for OSCC. Serum exoAlix was also identified as suitable for predicting therapeutic responses.
Objective Owing to variations in the exterior appearances of noncancerous diseases in the oral cavity, clinicians may have difficulty diagnosing oral squamous cell carcinoma (OSCC). Tissue biopsy is confirmatory, but invasive. Therefore, reliable tumor markers for OSCC are required. Here, exosomal Alix (exoAlix) levels were measured in serum/salivary samples from patients with OSCC and healthy controls (HCs). Methods Fifty-seven patients admitted to Nagoya University Hospital from 2017 through 2019 were enrolled, and serum samples (OSCC,n = 29; HC,n = 21) and/or saliva samples (OSCC,n = 23; HC,n = 20) were collected. Exosomal fractions were isolated using ultracentrifugation. ExoAlix levels were measured using enzyme-linked immunosorbent assay. Results Serum/salivary exoAlix levels were significantly higher in patients with OSCC than in HCs. Receiver operating characteristic analyses revealed that sensitivity, specificity, positive predictive value, and area under the curve were 0.345, 1.000, 1.000, and 0.685, respectively, for serum exoAlix and 0.348, 1.000, 1.000, and 0.712, respectively, for salivary exoAlix at optimal cut-off values (serum, 0.205; saliva, 0.193). All tested OSCC tissue sections (n = 21) were immuno-reactive for Alix. Conclusion Serum and salivary exoAlix were identified as potential diagnostic OSCC biomarkers. Serum exoAlix was suitable for prediction of therapeutic responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据