期刊
ORAL DISEASES
卷 27, 期 2, 页码 215-225出版社
WILEY
DOI: 10.1111/odi.13542
关键词
4-Nitroquinoline-1-oxide; high-fat diet; mouse model; oral cancer; oral squamous cell carcinoma
资金
- Midwestern University College of Graduate Studies
- College of Dental Medicine
High-fat diet and male sex worsen the pathology of 4-nitroquinoline-1-oxide-induced oral cancer, with elevated lymphoid cell infiltration contributing to disease progression.
Objective To compare the effects of dietary fat and sex on murine oral squamous cell carcinoma pathology. Materials and methods Male and female C57Bl/6 mice (36/sex) received a low-fat (10 kcal%) or high-fat (60 kcal%) diet. Water (control), vehicle, or 4-nitroquinoline-1-oxide in vehicle (50 mu g/ml) was provided for 17 weeks followed by six additional weeks of water. Oral lesion development was recorded weekly. Histopathologic changes in tongues were examined, and T cells (CD3+), macrophages (CD68+), and neutrophils (Ly6+) were quantified. Results All 4-nitroquinoline-1-oxide-treated mice developed oral tumors. High-fat diet exacerbated pathology, demonstrated by an increased final tumor burden (10.9 +/- 4.5 vs. 7.9 +/- 2.5, mm/mouse,p < .05; high-fat diet vs. low-fat diet, respectively), and a greater histopathology score. When dietary groups were combined, 4-nitroquinoline-1-oxide-treated males displayed higher histopathology scores than females (4.2 +/- 0.3 vs. 3.6 +/- 0.2, respectively,p < .05). Lymphoid cell infiltration was greater in the 4-nitroquinoline-1-oxide mouse tongues than controls: T cells (14.0 vs. 0.96 cells/mm(2)), macrophages (3.6 vs. 1.8 cells/mm(2)), and neutrophils (12.0 vs. 0.38 cells/mm(2)). Conclusion High-fat diet and male sex increased the pathology of 4-nitroquinoline-1-oxide-induced oral cancer. Elevated lymphoid cell infiltration contributed to disease pathology.
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