期刊
OPTICAL MATERIALS
卷 106, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.optmat.2020.109961
关键词
Biothiols; Detection methods; Fluorescence; Pattern recognition; Sensor
资金
- National Natural Science Foundation of China [81271639]
Biological thiols play essential roles in life activities. Detection and differentiation of different biothiols is of crucial need in biomedical research and diagnostics. Herein, a novel fluorescent sensor array was constructed to discriminate six kinds of biothiols including Cysteine (Cys), N-acetylcystein (NAC), 2-mercaptoethanol (MCE), Homocysteine (Hcy), Glutathione (GSH), and Glutathione disulfide (GSSG) simultaneously. This sensor array was fabricated by using three kinds of metal ions (i.e., Fe3+, Ni2+, and Cu2+)-N-CDs pairs. The N-CDs were synthesized with urea as nitrogen sources and folic acid as carbon sources via a simple microwave approach. Based on the competitive interactions of N-CDs with metal ions and thiols, the sensor array exhibits diverse fluorescence intensity response patterns, six thiols could be effectively and simultaneously differentiated in a wide concentration range (>= 50 mu M) by principal component analysis (PCA). Momver, double-blind samples of biothiols were recognized with 97.2% accuracy through linear discrimination analysis (LDA). More importantly, the sensor array showed good practicability in complex media such as fetal bovine serum, demonstrating this proposed sensor array held a great potential for the identification of biological thiols in real samples.
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