期刊
NEUROENDOCRINOLOGY
卷 111, 期 6, 页码 542-554出版社
KARGER
DOI: 10.1159/000509222
关键词
Tachykinin signaling; Preovulatory luteinizing hormone surge; Luteinizing hormone pulses
资金
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) [R01HD090151, R21HD095383]
- National Institute of Health (NIH)
- International Brain Research Organization (IBRO) Fellowship
- Lalor Foundation Research Fellowship
Tachykinins play an important role in the neuroendocrine control of reproduction by stimulating Kiss1 neurons to control GnRH pulsatility. Knockout mice for Tac1 (NKA/SP) and Tac2 (NKB) genes are fertile, suggesting compensatory mechanisms among different tachykinin systems. Further research confirmed the fundamental role of tachykinins in puberty onset, LH pulsatility, and facilitation of the preovulatory LH surge.
Tachykinins (neurokinin A [NKA], neurokinin B [NKB], and substance P [SP]) are important components of the neuroendocrine control of reproduction by direct stimulation of Kiss1 neurons to control GnRH pulsatility, which is essential for reproduction. Despite this role of tachykinins in successful reproduction, knockout (KO) mice for Tac1 (NKA/SP) and Tac2 (NKB) genes are fertile, resembling the phenotype of human patients bearing NKB signaling mutations, who often reverse their hypogonadal phenotype. This suggests the existence of compensatory mechanisms among the different tachykinin ligand-receptor systems to maintain reproduction in the absence of one of them. In order to test this hypothesis, we generated complete tachykinin-deficient mice (Tac1/Tac2KO). Male mice displayed delayed puberty onset and decreased luteinizing hormone (LH) pulsatility (frequency and amplitude of LH pulses) but preserved fertility. However, females did not show signs of puberty onset (first estrus) within 45 days after vaginal opening, they displayed a low frequency (but normal amplitude) of LH pulses, and 80% of them remained infertile. Further evaluation identified a complete absence of the preovulatory LH surge in Tac1/Tac2KO females as well as in wild-type females treated with NKB or SP receptor antagonists. These data confirmed a fundamental role of tachykinins in the timing of puberty onset and LH pulsatility and uncovered a role of tachykinin signaling in facilitation of the preovulatory LH surge. Overall, these findings indicate that tachykinin signaling plays a dominant role in the control of ovulation, with potential implications as a pathogenic mechanism and a therapeutic target to improve reproductive outcomes in women with ovulation impairments.
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