期刊
NATURE GENETICS
卷 52, 期 7, 页码 709-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41588-020-0645-y
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资金
- European Research Council [724824]
- Israel Science Foundation
- Chan Zuckerberg Initiative
- European Research Council (ERC) [724824] Funding Source: European Research Council (ERC)
Propagation of clonal regulatory programs contributes to cancer development. It is poorly understood how epigenetic mechanisms interact with genetic drivers to shape this process. Here, we combine single-cell analysis of transcription and DNA methylation with a Luria-Delbruck experimental design to demonstrate the existence of clonally stable epigenetic memory in multiple types of cancer cells. Longitudinal transcriptional and genetic analysis of clonal colon cancer cell populations reveals a slowly drifting spectrum of epithelial-to-mesenchymal transcriptional identities that is seemingly independent of genetic variation. DNA methylation landscapes correlate with these identities but also reflect an independent clock-like methylation loss process. Methylation variation can be explained as an effect of globaltrans-acting factors in most cases. However, for a specific class of promoters-in particular, cancer-testis antigens-de-repression is correlated with and probably driven by loss of methylation incis. This study indicates how genetic sub-clonal structure in cancer cells can be diversified by epigenetic memory. Longitudinal single-cell analysis of transcription and DNA methylation dynamics in cancer cell lines suggests a clonally stable epigenetic memory. Colon cancer cells show a spectrum of epithelial-to-mesenchymal identities that seems independent of genetic variation.
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