期刊
NATURE
卷 584, 期 7821, 页码 410-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-020-2461-z
关键词
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资金
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- National BioResource Project (NBRP)
- DZNE
- Max Planck Society
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [SFB 1035, DA 1906/4-1]
- Marie Curie International Reintegration Grant [322120]
- International C. elegans Gene Knockout Consortium
In metazoans, the secreted proteome participates in intercellular signalling and innate immunity, and builds the extracellular matrix scaffold around cells. Compared with the relatively constant intracellular environment, conditions for proteins in the extracellular space are harsher, and low concentrations of ATP prevent the activity of intracellular components of the protein quality-control machinery. Until now, only a few bona fide extracellular chaperones and proteases have been shown to limit the aggregation of extracellular proteins(1-5). Here we performed a systematic analysis of the extracellular proteostasis network in Caenorhabditis elegans with an RNA interference screen that targets genes that encode the secreted proteome. We discovered 57 regulators of extracellular protein aggregation, including several proteins related to innate immunity. Because intracellular proteostasis is upregulated in response to pathogens(6-9), we investigated whether pathogens also stimulate extracellular proteostasis. Using a pore-forming toxin to mimic a pathogenic attack, we found that C. elegans responded by increasing the expression of components of extracellular proteostasis and by limiting aggregation of extracellular proteins. The activation of extracellular proteostasis was dependent on stress-activated MAP kinase signalling. Notably, the overexpression of components of extracellular proteostasis delayed ageing and rendered worms resistant to intoxication. We propose that enhanced extracellular proteostasis contributes to systemic host defence by maintaining a functional secreted proteome and avoiding proteotoxicity.
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