期刊
NANO LETTERS
卷 20, 期 7, 页码 5228-5235出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c01503
关键词
Cell-membrane-camouflaged nanoparticles; AuNPs; plasmonic imaging; homotypic targeting; integrin alpha(v)beta(3)
类别
资金
- National Natural Science Foundation of China [31771102, 21974089, 91953016, 81773434, 21991134, 21834007]
- National Key RAMP
- D Program of China [2016YFA0400900]
- Major Science and Technology Innovation Program of Shanghai Municipal Education Commission [2019-01-07-00-01-E00059]
- Key Research Program of Frontier Sciences [QYZDJ-SSW-SLH031]
- Open Large Infrastructure Research of CAS, Chinese Academy of Sciences
- LU Jiaxi International Team of the Chinese Academy of Sciences
- K.C. Wong Foundation at Shanghai Jiao Tong University
Cell-membrane-camouflaged nanoparticles (CMCNPs) have been increasingly exploited to develop various therapeutic tools due to their high biocompatibility and cell-type-specific tumor-targeting properties. However, the molecular mechanism of CMCNPs for homotypic targeting remains elusive. Here, we develop a plasmonic imaging method by coating gold nanoparticles (AuNPs) with cancer cell membranes and perform plasmonic imaging of the interactions between CMC-NPs and living cells at the single-cell level. Quantitative analysis of CMC-NPs in a different clustering status reveals that the presence of cell membranes on CMC-NPs results in a 7-fold increase in homotypic cell delivery and nearly 2 orders of magnitude acceleration of the intracellular agglomeration process. Significantly, we identify that integrin alpha(v)beta(3), a cell surface receptor abundantly expressed in tumor cells, is critical for the selective cell recognition of CMC-NPs. We thus establish a single-cell plasmonic imaging platform for probing NP-cell interactions, which sheds new light on the therapeutic applications of CMC-NPs.
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