期刊
MYCORRHIZA
卷 30, 期 5, 页码 555-566出版社
SPRINGER
DOI: 10.1007/s00572-020-00976-6
关键词
Ectomycorrhiza; Monolignol; Root-knot nematode; Secondary metabolites; Transcriptomics; Transgenic poplar
资金
- Fonds de la Recherche Scientifique (FRS-FNRS) [T. 0004.14]
- Multidisciplinary Research Partnership Biotechnology for a sustainable economy
- European Commission's Directorate-General for Research within the 7th Framework Program (FP7/2007-2013) [270,089]
- French National Agency of Research (ANR) as part of the Investissement d'Avenir program of Labex ARBRE [ANR-11_LABX-0002-01, CFP15]
- US Department of Energy through the Oak Ridge National Laboratory Scientific Focus Area for Genomics Foundational Sciences (Plant Microbe Interfaces Project)
This study investigates the impact of the alteration of the monolignol biosynthesis pathway on the establishment of the in vitro interaction of poplar roots either with a mutualistic ectomycorrhizal fungus or with a pathogenic root-knot nematode. Overall, the five studied transgenic lines downregulated forcaffeoyl-CoAO-methyltransferase(CCoAOMT),caffeic acidO-methyltransferase(COMT),cinnamoyl-CoA reductase(CCR),cinnamyl alcohol dehydrogenase(CAD) or bothCOMTandCADdisplayed a lower mycorrhizal colonisation percentage, indicating a lower ability for establishing mutualistic interaction than the wild-type. The susceptibility to root-knot nematode infection was variable in the five lines, and the CAD-deficient line was found to be less susceptible than the wild-type. We discuss these phenotypic differences in the light of the large shifts in the metabolic profile and gene expression pattern occurring between roots of the CAD-deficient line and wild-type. A role of genes related to trehalose metabolism, phytohormones, and cell wall construction in the different mycorrhizal symbiosis efficiency and nematode sensitivity between these two lines is suggested. Overall, these results show that the alteration of plant metabolism caused by the repression of a single gene within phenylpropanoid pathway results in significant alterations, at the root level, in the response towards mutualistic and pathogenic associates. These changes may constrain plant fitness and biomass production, which are of economic importance for perennial industrial crops such as poplar.
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