期刊
MOLECULES
卷 25, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/molecules25122900
关键词
lactate dehydrogenase; Hoechst stain; granuloma tissue; pain; inflammatory mediators
资金
- South African Research Chairs Initiative of the Department of Science and Technology [98337]
- National Research Foundation of South Africa [98337]
- University of Johannesburg (URC)
- National Research Foundation (NRF)
- CSIR (Council for Scientific and industrial Research)-NLC (National Laser Centre) Laser Rental Pool Programme
Cancer, pain and inflammation have long been a cause for concern amongst patients, clinicians and research scientists. There is an alarming increase in the demand for medicines suppressing these disease conditions. The present study investigates the role ofSyzygium mundagambark methanol (SMBM) extract against MCF-7 breast cancer cells, pain and inflammation. The MCF-7 cells treated with SMBM were analyzed for adenosine triphosphate (ATP), lactate dehydrogenase (LDH) levels, changes in cell morphology and nuclear damage. Hot plate, acetic acid and formalin-induced pain models were followed to determine the analgesic activity. Anti-inflammatory activity was studied using carrageenan, egg albumin and cotton pellet induced rat models. Microscopic images of cells in SMBM treated groups showed prominent cell shrinkage and nuclear damage. Hoechst stain results supported the cell death morphology. The decline in ATP (47.96%) and increased LDH (40.96%) content indicated SMBM induced toxicity in MCF-7 cells. In the in vivo study, a higher dose (200 mg/kg) of the extract was found to be effective in reducing pain and inflammation. The results are promising and the action of the extract on MCF-7 cells, pain and inflammation models indicate the potential of drugs of natural origin to improve current therapies.
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