期刊
MOLECULAR MEDICINE REPORTS
卷 22, 期 3, 页码 2063-2075出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2020.11253
关键词
SH-SY5Y cells; alpha 7 nicotinic acetylcholine receptor; beta-amyloid peptide; synaptic protein; synaptophysin; apoptotic rate; synaptosomal-associated protein of 25 kDa; postsynaptic density of 95 kDa; Alzheimer's disease
资金
- Chinese National Natural Science Foundation [81860207]
- Program for Changjiang Scholars and Innovative Research Team in University [IR T13058]
- Guizhou Foundation [(2014)06, 2014(6008)]
- Guizhou Science and Technology Foundation [(2019)1437]
- Guizhou Province Graduate Education Innovation Program Foundation [YJSCXJH(2018) 040, YJSCXJH(2019)070]
Alzheimer's disease (AD) is a chronic neurodegenerative, and abnormal aggregation of the neurotoxic beta amyloid (A beta) peptide is an early event in AD. The present study aimed to determine the correlation between the nicotinic acetylcholine receptor alpha 7 subunit (alpha 7 nAChR) and A beta in the brains of patients with AD, and to investigate whether the increased expression levels of the alpha 7 nAChR could alter the neurotoxicity of A beta. The expression levels of alpha 7 nAChR and A beta in the brains of patients with AD and healthy brains were analyzed using immunofluorescence. Moreover, SH-SY5Y cells were used to stably overexpress or silence alpha 7 nAChR expression levels, prior to the treatment with or without 1 mu mol/l A beta(1-42) oligomer (A beta O). The mRNA and protein expression levels of alpha 7 nAChR, synaptophysin (SYP), postsynaptic density of 95 kDa (PSD-95) and synaptosomal-associated protein of 25 kDa (SNAP-25) were subsequently analyzed using reverse transcription-quantitative PCR and western blotting. In addition, the concentration of acetylcholine (ACh) and the activity of acetylcholinesterase (AChE) were analyzed using spectrophotometry, while the cell apoptotic rate was determined using flow cytometry. The expression of A beta in the brains of patients with AD was found to be significantly increased, whereas the expression of alpha 7 nAChR was significantly decreased compared with the healthy control group. In vitro, the expression levels of alpha 7 nAChR were significantly increased or decreased following the overexpression or silencing of the gene, respectively. Consistent with these observations, the mRNA and protein expression levels of SYP, PSD-95 and SNAP-25 were also significantly increased following the overexpression of alpha 7 nAChR and decreased following the genetic silencing of the receptor. In untransfected or negative control cells, the expression levels of these factors and the apoptotic rate were significantly reduced following the exposure to A beta O, which was found to be attenuated by alpha 7 nAChR overexpression, but potentiated by alpha 7 nAChR RNA silencing. However, no significant differences were observed in either the ACh concentration or AChE activity following transfection. Collectively, these findings suggested that alpha 7 nAChR may protect the brains of patients with AD against A beta, as alpha 7 nAChR overexpression increased the expression levels of SYP, SNAP-25 and PSD-95, and attenuated the inhibitory effect of A beta on the expression of these synaptic proteins and cell apoptosis. Overall, this indicated that alpha 7 nAChR may serve an important neuroprotective role in AD.
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