期刊
MOLECULAR ECOLOGY
卷 30, 期 13, 页码 3289-3298出版社
WILEY
DOI: 10.1111/mec.15594
关键词
amphibians; archival DNA; biomonitoring; endangered species; metabarcoding; population declining
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2012/25370-2, 2013/50741-7, 2016/14054-3]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [302518/2013-4, 401729/2013-3, 589/2016-0306623/2018-8]
The study successfully used environmental DNA (eDNA) metabarcoding to detect DNA traces of 30 target amphibian species in Brazil, including four declined species, two locally disappeared species, and one species not seen since 1968. This highlights the potential of eDNA in biodiversity monitoring and conservation biology for evaluating the persistence and distribution of threatened species.
Declines and extinctions are increasing globally and challenge conservationists to keep pace with biodiversity monitoring. Organisms leave DNA traces in the environment, e.g., in soil, water, and air. These DNA traces are referred to as environmental DNA (eDNA). The analysis of eDNA is a highly sensitive method with the potential to rapidly assess local diversity and the status of threatened species. We searched for DNA traces of 30 target amphibian species of conservation concern, at different levels of threat, using an environmental DNA metabarcoding approach, together with an extensive sequence reference database to analyse water samples from six montane sites in the Atlantic Coastal Forest and adjacent Cerrado grasslands of Brazil. We successfully detected DNA traces of four declined species (Hylodes ornatus,Hylodes regius,Crossodactylus timbuhy, andVitreorana eurygnatha); two locally disappeared (Phasmahyla exilisandPhasmahyla guttata); and one species that has not been seen since 1968 (putatively assigned toMegaelosia bocainensis). We confirm the presence of species undetected by traditional methods, underscoring the efficacy of eDNA metabarcoding for biodiversity monitoring at low population densities, especially in megadiverse tropical sites. Our results support the potential application of eDNA in conservation biology, to evaluate persistence and distribution of threatened species in surveyed habitats or sites, and improve accuracy of red lists, especially for species undetected over long periods.
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