4.7 Article

Voltammetric sensing of recombinant viral dengue virus 2 NS1 based on Au nanoparticle-decorated multiwalled carbon nanotube composites

期刊

MICROCHIMICA ACTA
卷 187, 期 6, 页码 -

出版社

SPRINGER WIEN
DOI: 10.1007/s00604-020-04339-y

关键词

Electrochemical biosensor; Differential pulste voltammetry; Carbon nanotube; Gold nanoparticles; Dengue toxin

资金

  1. Uppsala University
  2. Swedish Strategic Research Foundation [SSF FFL15-0174]
  3. Swedish Research Council [VR 2014-5588]
  4. Wallenberg Academy Fellow Program
  5. French grant Labex ARCANE [ANR-17-EURE-0003]
  6. French grant CBH-EUR-GS [ANR-17-EURE-0003]

向作者/读者索取更多资源

A homemade gold electrode is modified with a carbon nanotubes/gold nanoparticles nanocomposite to perform selective and sensitive electrochemical detection of dengue toxin. This nanostructured composite offers a large specific surface and a reactive interface allowing the immobilization of biological material. Dengue antibodies are immobilized on gold nanoparticles via covalent bonding for dengue toxin detection. The porous tridimensional network of carbon nanotubes and gold nanoparticles enhances the electrochemical signal and the overall performance of the sensor. After optimization, the system exhibits a high sensitivity of - 0.44 +/- 0.01 mu A per decade with wide linear range between 1 x 10(-12) and 1 x 10(-6) g/mL at a working potential of 0.22 V vs Ag/AgCl. The extremely low detection limit (3 x 10(-13) g/mL) ranks this immunosensor as one of the most efficient reported in the literature for the detection of recombinant viral dengue virus 2 NS1. This biosensor also offers good selectivity, characterized by a low response to various non-specific targets and assays in human serum. The outstanding performances and the reproducibility of the system place the biosensor developed among the best candidates for future medical applications and for early diagnosis of dengue fever.

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