4.2 Article

Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii

期刊

MICROBIAL DRUG RESISTANCE
卷 27, 期 3, 页码 328-336

出版社

MARY ANN LIEBERT, INC
DOI: 10.1089/mdr.2020.0188

关键词

Acinetobacter baumannii; environment; colistin; resistance; wastewater

资金

  1. Ministry of Education, Science and Technological Development of the Republic of Serbia
  2. Croatian Science Foundation [IP-2014-09-5656]

向作者/读者索取更多资源

The molecular mechanisms of colistin resistance in environmental isolates of A. baumannii were investigated in this study, revealing nucleotide variations resulting in amino acid changes and increased levels of eptA mRNA. Isolates with colistin and pandrug resistance could potentially be a source for serious community-acquired infections, as they showed excellent survival in river water.
Although the molecular mechanisms of carbapenem resistance of environmental isolates ofAcinetobacter baumanniiare well described, data on the mechanisms of colistin resistance are scarce. In this study, we report the molecular mechanisms of colistin resistance in environmental isolates ofA. baumannii. Seven clinically relevant isolates ofA. baumanniibelonging to ST-2(Pasteur)were recovered from hospital wastewater and wastewater treatment plant. The phenotypic resistance to colistin was confirmed by broth microdilution with minimum inhibitory concentration values ranging from 20 to 160 mg/L. Colistin sulfate and colistimethate sodium showed bactericidal activity against two colistin-heteroresistant isolatesin vitro, but substantially recovery of population was observed after prolonged incubation.In silicogenome analysis revealed nucleotide variations resulting in amino acid changes in LpxC (N286D), LpxD (E117K), PmrB (A138T, R263S, L267W, Q309P, and A444V), and EptA (F166L, I228V, R348K, A370S, and K531T). According to reverse transcription quantitative PCR, all isolates had increased levels ofeptAmRNA and decreased levels oflpxAandlpxDmRNA. Isolates expressed low hydrophobicity, biofilm, and pellicle formation, but showed excellent survival in river water during 50 days of monitoring. Colistin- and pandrug-resistantA. baumanniidisseminated in the environment could represent the source for the occurrence of serious community-acquired infections.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.2
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据