期刊
MATRIX BIOLOGY
卷 94, 期 -, 页码 1-17出版社
ELSEVIER
DOI: 10.1016/j.matbio.2020.06.004
关键词
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资金
- French Agence Nationale de la Recherche [ANR-08-PCVI-0031, ANR-13-RPIB-0003-01]
- Ligue Nationale Contre le Cancer
- CNRS in the framework of the GDR GAG [GDR 3739]
- Region Rhone-Alpes [ARC 2]
- [UMS344/US8]
- Agence Nationale de la Recherche (ANR) [ANR-08-PCVI-0031] Funding Source: Agence Nationale de la Recherche (ANR)
Re-epithelialization describes the resurfacing of a skin wound with new epithelium. In response to various stimuli including that of growth factors, cytokines and extracellular matrix (ECM), wound edge epidermal keratinocytes undergo cytoskeleton rearrangements compatible with their motile behavior and develop protrusive adhesion contacts. Matrix metalloproteinases (MMP) expression is crucial for proper cell movement and ECM remodeling; however, their deposition mechanism is unknown in keratinocytes. Here, we show that similar to cytokine IL-1 beta, the precursor laminin 332 pro-migratory fragment G45 induces expression of the MMP-9 pro-enzyme, which together with MMP-14, further exerts its proteolytic activity within epithelial podosomes. This event strictly depends on the expression of the proteoglycan receptor syndecan-1 that was found in a ring surrounding the podosome core, co-localised with CD44. Our findings uncover that by directly recruiting both syndecan-1 and CD44, the laminin-332 G45 domain plays a major role in regulating mechanisms underlying keratinocyte / ECM remodeling during wound repair. (C) 2020 The Author(s). Published by Elsevier B.V.
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