期刊
MARINE DRUGS
卷 18, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/md18060316
关键词
ROS; Phaeophyta; carbohydrate; UVB irradiation; HaCaT cells; zebrafish
资金
- National Key R&D Program of China [2018YFD0901104]
- Major Scientific and Technological Innovation Project of Shandong Province [2019JZZY020613]
A fucose-rich fucoidan was purified from brown seaweedSaccharina japonica, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry.
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