期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 135, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2020.110059
关键词
beta-carotene; Nanoemulsions; In vitro digestion; Intestinal barrier
资金
- Fondo Europeo de Desarrollo Regional (FEDER)
- Ministerio de Economia y Competitividad [AGL2015-65975-R]
- Enterprise Ireland (Career-FIT)
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [713654, MF2018-0151 Food-BIBS]
- Science Foundation Ireland [16/RC/3835]
- Agencia de Gestio d'Ajuts Universitaris I de Recerca (AGAUR) from the Catalan government (Spain)
The study found that the use of nanoemulsions stabilized with lecithin can enhance the bioavailability and cellular content of beta-carotene in the intestine, while nanoemulsions stabilized with sodium caseinate perform worse in comparison. Additionally, the emulsifiers in the nanoemulsions also impact the permeability and delivery of beta-carotene.
To increase the intestinal delivery of dietary beta-carotene, there is a need to develop nanostructured food systems to encapsulate this fat soluble bioactive. The aim of this study was to evaluate the bioacessibility and bioavailability across the intestinal barrier of beta-carotene-enriched nanoemulsions stabilised with two emulsifiers (lecithin or sodium caseinate) by coupling an in vitro gastrointestinal digestion with two in vitro cell culture models (Caco-2 or co-culture of Caco-2/HT29-MTX). Nanoemulsions stabilised with lecithin had significantly higher beta-carotene in the gastrointestinal digested micellar fraction, lower beta-carotene in the Caco-2 (and Caco-2/HT29-MTX) apical compartment and significantly higher beta-carotene in Caco-2 cellular content compared to beta-carotene-enriched nanoemulsions stabilised with sodium caseinate. Finally, to assess anti-inflammatory activity of digested nanoemulsions, lipopolysaccharide stimulated macrophages were exposed to Caco-2 basolateral samples with levels of TNF-alpha and IL-beta, subsequently quantified. A TNF-alpha response from stimulated THP-1 macrophages was elicited by basolateral samples, regardless the emulsifier used to formulate nanoemulsions. This study demonstrated that beta-carotene permeability is influenced by the food derived emulsifier used for stabilising nanoemulsions, indicating that composition may be a critical factor for beta-carotene delivery.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据