期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 134, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2020.109945
关键词
E. coli O157:H7; Mixed biofilm; Bacteriophage
资金
- JSPS KAKENHI [JP19H02912]
This study describes the characterization of bacteriophage FP43, active against pathogenic and non-pathogenic strains of Escherichia coil, and its ability to inhibit and remove a mixed biofilm of enterohemorrhagic E. coli O157: H7 and O91:H-. Phage FP43 is a member of the Myoviridae family, having a dsDNA genome consisting of 169,248 bp with good stability. It has a short latent period and large burst size. Phage FP43 decreased the formation of biofilm, comprising E. coli O157:H7 and O91:H-, by 82.4% at 30 degrees C. After incubation for 6 h with FP43, viable counts of E. coli O157:H7 and total cells in the biofilm were reduced by 2.76 and 2.85 log, respectively. In planktonic cells, viable E. coli O157:H7 and total counts decreased by 3.44 and 3.62 log, respectively, after incubation with the phage for 4 h. Moreover, more than 60% of an established, mixed biofilm was removed after a 6 h exposure to the phage, in which E. coli O157:H7 and total viable counts decreased by 2.07 and 1.93 log, respectively. These results suggest that bacteriophage FP43 has broad host range against both pathogenic and non-pathogenic E. coil, and potential to reduce viable counts of both entemhemorrhagic E. coli O157:H7 and E. coli O91 in mixed-strain biofilm.
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