期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 127, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2020.109382
关键词
Thymol; Carvacrol; Encapsulation; Disinfection; Salmonella
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS)
Nanoliposome-encapsulated and free thymol, carvacrol, and thymol/carvacrol (1:1) were assessed as antimicrobials against a Salmonella pool. Liposome-encapsulated thymol (TL), carvacrol (CL) and thymol/carvacrol (TCL) presented 230-270 nm diameter, satisfactory polydispersity (0.24-0.31), zeta potential of approximately -30 mV and high encapsulation efficiencies (similar to 99%), remaining stable during 28 days at 4 degrees C. Minimum inhibitory concentrations (MIC) of free antimicrobials were 331 mu g/mL. Encapsulation increased MIC to 663 (TL, CL) and 1325 mu g/mL (TCL). Time-kill curves with antimicrobial preparations (265.0 mu g/mL) demonstrated superior activities for free antimicrobials; among nanoliposomes, TCL exhibited higher activity. At MIC, antimicrobials impeded bacterial adhesion to glass. Without antimicrobials, Salmonella (similar to 8 log Colony Forming Units (CFU)/mL) adhered to glass reaching 5.89 log CFU/cm(2) after 15 min. Inactivation of glass-attached Salmonella was complete following 1-min treatments with free (MIC, 2 x MIC) or encapsulated (2 x MIC) antimicrobials. At MIC, antimicrobial-loaded nanoliposomes decreased adhered populations by similar to 3.9 log CFU/cm(2); at 0.5 x MIC, reductions of 1.93 (CL) and 1.58 log CFU/cm(2) (TCL) were observed. Free antimicrobials were more effective against planktonic and sessile Salmonella at contact times employed in food processing environments. Nanoliposome-encapsulated natural antimicrobials warrant investigations on surface disinfection schemes and incorporation into foods.
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