期刊
LUMINESCENCE
卷 35, 期 8, 页码 1296-1303出版社
WILEY
DOI: 10.1002/bio.3891
关键词
biothiol detection; gold nanoclusters-Hg(II) system; Hg(II)-thiol coordination complex; turn-on fluorescent assay
资金
- 1000 Talents Program of Zhongyuan
- Plan for Young Excellent Teachers in Universities of Henan Province [2017GGJS100]
- National Natural Science Foundation of China [U1704153]
- Nanhu Scholars Program for Young Scholars of XYNU
Fluorescent adenine (A)-rich DNA-templated gold nanoclusters were demonstrated to be a novel probe for determination of biothiols (including cysteine, glutathione, and homocysteine). Fluorescence intensity of adenine-rich DNA-templated gold nanoclusters could be greatly quenched by Hg(II) ions through the formation of a gold nanoclusters-Hg(II) system. When biothiols (cysteine as the model) were introduced into the system, the fluorescence intensity recovered due to the formation of a more stable Hg(II)-thiol coordination complex using Hg-S metal-ligand bonds, which inhibited the Hg(II)-mediated fluorescence quenching of adenine-rich DNA-templated gold nanoclusters. Based on this fluorescence phenomenon, an on-off-on fluorescence strategy was designed for the sensitive determination of biothiols. The method allowed sensitive detection of cysteine with a linear detection range from 100 nM to 5 mu M and a limit of detection of 30 nM. Additionally, the assay can be applied for detection of biothiol levels in human plasma samples. Therefore, it can provide a simple and rapid fluorescent platform for biothiol detection.
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