期刊
JOURNAL OF VIROLOGICAL METHODS
卷 282, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2020.113888
关键词
Covid-19; SARS-CoV-2; next-generation sequencing; metatranscriptomics
资金
- Universal CNPq-2019
- [MCTI/FINEP/CT-INFRA -01/2013]
- [MCTI/FINEP/FNDCT 01/2016 -Zika]
Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
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