Neutrophil Gelatinase?Associated Lipocalin Protects from ANCA-Induced GN by Inhibiting T(H)17 Immunity

Significance Statement Neutrophil gelatinase?associated lipocalin (NGAL) is produced by injured renal cells and by neutrophils that are central to ANCA-associated vasculitis. The authors show that circulating and urinary NGAL is not only a marker for ANCA-induced necrotizing crescentic GN, but also that neutrophil NGAL is involved mechanistically in ANCA-associated vasculitis. They demonstrate that ANCA-activated neutrophils release NGAL, and that chimeric mice deficient in neutrophil-derived NGAL develop accelerated myeloperoxidase-ANCA?induced crescentic GN, with increased renal CD4(+) T cells?particularly T helper 17 (T(H)17) cells?acting as mediators of the accelerated phenotype. They also demonstrated that iron siderophore?loaded NGAL suppresses T(H)17 polarization. Their findings indicate that bone marrow?derived NGAL, presumably from neutrophils, protects from ANCA-induced necrotizing and crescentic GN by downregulating T(H)17 immunity. Background Neutrophil gelatinase?associated lipocalin (NGAL) is a diagnostic marker of intrinsic kidney injury produced by damaged renal cells and by neutrophils. ANCA-associated vasculitis features necrotizing crescentic GN (NCGN), and ANCA-activated neutrophils contribute to NCGN. Whether NGAL plays a mechanistic role in ANCA-associated vasculitis is unknown. Methods We measured NGAL in patients with ANCA-associated vasculitis and mice with anti-myeloperoxidase (anti-MPO) antibody?induced NCGN. We compared kidney histology, neutrophil functions, T cell proliferation and polarization, renal infiltrating cells, and cytokines in wild-type and NGAL-deficient chimeric mice with anti-MPO antibody?induced NCGN. To assess the role of T(H)17 immunity, we transplanted irradiated MPO-immunized MPO-deficient mice with bone marrow from either wild-type or NGAL-deficient mice; we also transplanted irradiated MPO-immunized MPO/IL-17A double-deficient mice with bone marrow from either IL-17A?deficient or NGAL/IL-17A double-deficient mice. Results Mice and patients with active ANCA-associated vasculitis demonstrated strongly increased serum and urinary NGAL levels. ANCA-stimulated neutrophils released NGAL. Mice with NGAL-deficient bone marrow developed worsened MPO-ANCA?induced NCGN. Intrinsic neutrophil functions were similar in NGAL-deficient and wild-type neutrophils, whereas T cell immunity was increased in chimeric mice with NGAL-deficient neutrophils with more renal infiltrating T(H)17 cells. NGAL-expressing neutrophils and CD3(+) T cells were in close proximity in kidney and spleen. CD4(+) T cells showed no intrinsic difference in proliferation and polarization in vitro, whereas iron siderophore?loaded NGAL suppressed T(H)17 polarization. We found significantly attenuated NCGN in IL-17A?deficient chimeras compared with MPO-deficient mice receiving wild-type bone marrow, as well as in NGAL/IL-17A?deficient chimeras compared with NGAL-deficient chimeras. Conclusions Our findings support that bone marrow?derived, presumably neutrophil, NGAL protects from ANCA-induced NCGN by downregulating T(H)17 immunity.