期刊
CLINICAL INFECTIOUS DISEASES
卷 64, 期 6, 页码 711-718出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/ciw805
关键词
colistin; carbapenem-resistant Enterobacteriaceae; Klebsiella pneumoniae; mortality; ST258
资金
- National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) [UM1AI104681, R21AI114508]
- Clinical and Translational Science Collaborative of Cleveland from the National Center for Advancing Translational Sciences component of the NIH [UL1TR000439]
- NIH Roadmap for Medical Research
- NIH [K24-AI093969, R01AI104895, R21AI107302]
- NIAID of the NIH [R21AI114508, R01AI100560, R01AI063517, R01AI072219]
- Cleveland Department of Veterans Affairs [1I01BX001974]
- Geriatric Research Education and Clinical Center VISN from Biomedical Laboratory Research & Development Service of the Veterans Affairs Office of Research and Development [10]
- Research Program Committees of the Cleveland Clinic
- STERIS Corporation
- NIAID, Division of Microbiology and Infectious Diseases [protocol 10-0065, R01AI119446-01]
Background. Polymyxins including colistin are an important last-line treatment for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKp). Increasing use of colistin has led to resistance to this cationic antimicrobial peptide. Methods. A cohort nested within the Consortium on Resistance against Carbapenems in Klebsiella pneumoniae (CRACKLE) was constructed of patients with infection, or colonization with CRKp isolates tested for colistin susceptibility during the study period of December, 2011 to October, 2014. Reference colistin resistance determination as performed by broth macrodilution was compared to results from clinical microbiology laboratories (Etest) and to polymyxin resistance testing. Each patient was included once, at the time of their first colistin-tested CRKp positive culture. Time to 30-day in-hospital all-cause mortality was evaluated by Kaplan-Meier curves and Cox proportional hazard modeling. Results. In 246 patients with CRKp, 13% possessed ColR CRKp. ColR was underestimated by Etest (very major error rate = 35%, major error rate = 0.4%). A variety of rep-PCR strain types were encountered in both the ColS and the ColR groups. Carbapenem resistance was mediated primarily by bla(KPC-2) (46%) and bla(KPC-3) (50%). ColR was associated with increased hazard for in-hospital mortality (aHR 3.48; 95% confidence interval, 1.73-6.57; P < .001). The plasmid-associated ColR genes, mcr-1 and mcr-2 were not detected in any of the ColR CRKp. Conclusions. In this cohort, 13% of patients with CRKp presented with ColR CRKp. The apparent polyclonal nature of the isolates suggests de novo emergence of ColR in this cohort as the primary factor driving ColR. Importantly, mortality was increased in patients with ColR isolates.
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