期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 142, 期 25, 页码 10894-10898出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b11962
关键词
-
资金
- National Key Research and Development Project [2016YFA0501500]
- National Natural Science Foundation of China [21925701, 91953109, 21521004]
Itaconate is an anti-inflammatory metabolite involved in pathogen-macrophage interactions, but the mechanisms underlying its effect are not fully understood. Competitive cysteine profiling has been performed to interrogate itaconate's reactivity in cell lysates, but methods for analyzing targets of itaconation directly in living macrophages are still lacking. In this work, we developed a specific bioorthogonal probe, itaconate-alkyne (ITalk), for quantitative and site-specific chemoproteomic profiling of itaconation in inflammatory macrophages. ITalk recapitulates the anti-inflammatory property of itaconate and enables biochemical evaluation and proteomic analysis of its direct targets. Our study delineates the widespread landscape of itaconate substrates, providing a versatile tool and comprehensive resource for investigating its function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据