期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 142, 期 31, 页码 13350-13355出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.0c06866
关键词
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资金
- Air Force Office of Scientific Research Award [FA9550-17-1-0348]
- Center for Bio-Inspired Energy Science, an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Basic Energy Sciences Award [DE-SC0000989]
- National Cancer Institute of the National Institutes of Health [U54CA199091]
- Air Force Research Laboratory [FA8650-15-2-5518]
- Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF) [ECCS1542205]
- State of Illinois
- International Institute for Nanotechnology (IIN)
- Northwestern University - VFlow Cytometry Core Facility - Cancer Center Support Grant (NCI) [CA060553]
- Chicago Cancer Baseball Charities
- H Foundation at the Lurie Cancer Center of Northwestern University
We report the development of a new strategy for the chemical analysis of live cells based on protein spherical nucleic acids (ProSNAs). The ProSNA architecture enables analyte detection via the highly programmable nucleic acid shell or a functional protein core. As a proof-of-concept, we use an i-motif as the nucleic acid recognition element to probe pH in living cells. By interfacing the i-motif with a forced-intercalation readout, we introduce a quencher-free approach that is resistant to false-positive signals, overcoming limitations associated with conventional fluorophore/quencher-based gold NanoFlares. Using glucose oxidase as a functional protein core, we show activity-based, amplified sensing of glucose. This enzymatic system affords greater than 100-fold fluorescence turn on in buffer, is selective for glucose in the presence of close analogs (i.e., glucose-6-phosphate), and can detect glucose above a threshold concentration of similar to 5 mu M, which enables the study of relative changes in intracellular glucose concentrations.
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