期刊
JOURNAL OF PROTEOMICS
卷 221, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jprot.2020.103683
关键词
Metabolic healthy obesity (MHO); Non-alcoholic fatty liver disease (NAFLD); TMT quantitative proteomics
资金
- National Natural Science Foundation of China [31371373, 31771572, 31300103]
- Nature Science Foundation of Jiangsu Province [BK20191356]
- Open Fund of State Key Laboratory of Natural Medicines [SKLNMKF201811]
- Six talent peaks project in Jiangsu Province [yy-014]
- Research of Institute of Hospital Management Nanjing University [NDYG2017016]
- Innovation Capability Development Project of Jiangsu Province [BM2015004]
- Nanjing Healthy and Family Planning Commission Medical Science Technology Innovation Platform Project [ZDX16006]
- National Human Genetic Resources Sharing Service Platform [2005DKA21300]
Obese subjects with non-alcoholic fatty liver disease (NAFLD) and considered metabolically healthy have not been well differentiated. In this study, obese subjects were divided into metabolic healthy obesity (MHO) and NAFLD groups. Liver tissues were sampled from these two types of subjects undergoing bariatric surgery, and proteins in the liver tissues that expressed differently between the two groups of subjects were identified by Tandem Mass Tags (TMT) assay. Compared with the MHO group, 132 proteins were found to be upregulated and 84 proteins were found to be downregulated (mainly localized in mitochondria) in NAFLD group. The KEGG pathway analysis showed that significantly upregulated metabolic pathways include PPAR signaling, ECM-receptor interaction and oxidative phosphorylation was significantly downregulated. The GO analysis revealed that upregulated proteins were involved in extracellular structure organization, extracellular matrix organization and downregulated proteins took part in the oxidation-reduction process and so on. FBLN5 and DHRS2 were further validated by Western blot, immunohistochemistry and ELISA. All results demonstrate that FBLN5 expression was significantly upregulated but DHRS2 was significantly downregulated. Significance: The variation between MHO and NAFLD was studied by mass spectroscopy to evaluate the mechanism with which MHO subjects resist the harmful effects induced by obesity.
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