期刊
JOURNAL OF PROTEOMICS
卷 224, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jprot.2020.103842
关键词
Mycotoxins; Zearalenone; Intestine; Pig; Estrogen receptors; Proteomics
资金
- European Union [722634]
- Agence Nationale de la Recherche (ANR) [ANR-17-Carn012]
- Region Occitanie, European funds (Fonds Europeens de Developpement Regional, FEDER), Toulouse Metropole
- French Ministry of Research with the Investissement d'Avenir Infrastructures Nationales en Biologie et Sante program (ProFI, Proteomics French Infrastructure project) [ANR-10-INBS-08]
Intestinal epithelial homeostasis is regulated by a complex network of signaling pathways. Among them is estrogen signaling, important for the proliferation and differentiation of epithelial cells, immune signaling and metabolism. The mycotoxin zearalenone (ZEN) is an estrogen disruptor naturally found in food and feed. The exposure of the intestine to ZEN has toxic effects including alteration of the immune status and is possibly implicated in carcinogenesis, but the molecular mechanisms linked with these effects are not clear. Our objective was to explore the proteome changes induced by a short, non-cytotoxic exposure to ZEN in the intestine using pig jejunal explants. Our results indicated that ZEN promotes little proteome changes, but significantly related with an induction of ERa signaling and a consequent disruption of highly interrelated signaling cascades, such as NF-kappa B, ERK1/2, CDX2 and HIF1 alpha. The toxicity of ZEN leads also to an altered immune status characterized by the activation of the chemokine CXCR4/SDF-1 axis and an accumulation of MHC-I proteins. Our results connect the estrogen disrupting activity of ZEN with its intestinal toxic effect, associating the exposure to ZEN with cell-signaling disorders similar to those involved in the onset and progression of diseases such as cancer and chronic inflammatory disorders. Significance: The proteomics results presented in our study indicate that the endocrine disruptor activity of ZEN is able to regulate a cascade of highly inter-connected signaling events essential for the small intestinal cryptvillus cycle and immune status. These molecular mechanisms are also implicated in the onset and progress of intestinal immune disorders and cancer indicating that exposure to ZEN could play an important role in intestinal pathogenesis.
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