期刊
JOURNAL OF PROTEOME RESEARCH
卷 19, 期 11, 页码 4389-4392出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.0c00280
关键词
COVID-19; mass spectrometry; nucleoprotein; SARS-CoV-2
资金
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [RTG 2467, 391498659]
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据