期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 187, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2020.113293
关键词
Chaigui granules; Chemical constituents; Absorption; UHPLC-Q-orbitrap-MS/MS; Microdialysis; Everted gut sac
资金
- Ministry of Science and Technology [2017ZX09301-047]
- Social Development Projects of Key R&D Programs in Shanxi Province [201803D31019]
- Shanxi Key Laboratory of Active constituents Research and Utilization of TCM [201605D111004]
- Science and technology innovation team of Shanxi Province [201605D131045-18]
- Fund for Shanxi 1331 Project Key Collaborative Innovation Center
- Natural Science Foundation of State [81473415]
Established qualitative analysis method for Chaigui granules based on UHPLC-Q-orbitrap-MS/MS and applied to its absorption properties studies. The LC-MS method was established to identify the structures of the components and metabolites. And then biosamples of rats after administration, e.g. intestinal solution, serum and brain microdialysate, were detected in rats with same method. Xcalibur 3.2 software was used for mass spectrum analysis and identification. Compound discover 3.0 was used for metabolite analysis. 95 chemical constituents were identified from Chaigui granules, including sesquiterpenes, flavonoids, lactones, tannins, organic acids, saponins and so on. 82 components and 11 metabolites were found in intestinal solution. 28 chemical constituents and 32 metabolites were found in serum. 15 chemical constituents were found in brain microdialysate. Vanillic acid, abiflorin, paeoniflorin, 4-hydroxybenzoic acid, lactiflorin, Z-butylidenephthalide, saikosaponin c, saikosaponin a, atractylenolide III, saikosaponin g, saikosaponin b1, sesquiterpenes, butylphthalide, saikosaponin d and glycyrrhetinic acid directly passed through the blood-brain barrier, which might be speculated that Chaigui granule plays an antidepressant role mainly through regulating brain central mechanism and endocrine mechanism, and so on. It is a systematically applicable approach for rapid identification and relative quantitation of Chaigui granules in vivo by UHPLC-Q-orbitrap-MS/MS, provides an important basis for the safety evaluation and rational clinical application of Chaigui granules. (C) 2020 Elsevier B.V. All rights reserved.
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