期刊
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
卷 63, 期 3, 页码 199-215出版社
IOS PRESS
DOI: 10.3233/CH-162044
关键词
Humans; nitric oxide; normobaric hypoxia; red blood cells; red blood cell deformability; red blood cell-nitric oxide synthase
The aim was to study impacts of mild to severe hypoxia on human red blood cell (RBC)-nitric oxide synthase (NOS)-dependent NO production, protein S-nitrosylation and deformability. Ambient air oxygen concentration of 12 healthy subjects was step-wisely reduced from 20.95% to 16.21%, 12.35%, 10% and back to 20.95%. Additional in vitro experiments involved purging of blood (+/-sodium nitrite) with gas mixtures corresponding to in vivo intervention. Vital and hypoxia-associated parameters showed physiological adaptation to changing demands. Activation of RBC-NOS decreased with increasing hypoxia. RBC deformability, which is influenced by RBC-NOS activation, decreased under mild hypoxia, but surprisingly increased at severe hypoxia in vivo and in vitro. This was causatively induced by nitrite reduction to NO which increased S-nitrosylation of RBC alpha- and beta-spectrins - a critical step to improve RBC deformability. The addition of sodium nitrite prevented decreases of RBC deformability under hypoxia by sustaining S-nitrosylation of spectrins suggesting compensatory mechanisms of non-RBC-NOS-produced NO. The results first time indicate a direct link between maintenance of RBC deformability under severe hypoxia by nonenzymatic NO production because RBC-NOS activation is reduced. These data improve our understanding of physiological mechanisms supporting adequate blood and, thus, oxygen supply to different tissues under severe hypoxia.
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